As expected, flagellin induced the synthesis of the proinflammatory cytokines interleukin (IL)-8 and IL-6 (Supplementary Figure 2A, B) both in Calu-3-CFTR-WT and Calu-3-CFTR-KD cells, and this inflammatory response was relatively higher in the Calu-3-CFTR-KD cells, which agrees with previous work showing that CF epithelial cells from human (19) or porcine (20) origin exhibit an enhanced inflammatory response to flagellin. This evidence concerns the gene IL6 and cystic fibrosis.