As expected, flagellin induced the synthesis of the proinflammatory cytokines interleukin (IL)-8 and IL-6 (Supplementary Figure 2A, B) both in Calu-3-CFTR-WT and Calu-3-CFTR-KD cells, and this inflammatory response was relatively higher in the Calu-3-CFTR-KD cells, which agrees with previous work showing that CF epithelial cells from human (19) or porcine (20) origin exhibit an enhanced inflammatory response to flagellin. The gene discussed is CXCL8; the disease is cystic fibrosis.