As GFAP overexpression is considered a main pathogenetic determinant in AxD leading to GFAP accumulation, and the small heat shock proteins (sHSPs) overexpression a potential endogenous tool to counteract GFAP misfolding and cellular protein accumulation, the expression of GFAP and alphaB-Crystallin has been investigated in U251-MG cells after 24 and 48 h treatments. This evidence concerns the gene GFAP and Alexander disease.