As such, we targeted the expression of five genes with roles in HIV-1 infection (CD4, CXCR4, PSGL-1, TRIM5α and CPSF6) as well as CD46, in part using gRNAs pre-tested for each target for the efficiency of editing (Extended Data Fig. 4a) and protein loss was assessed in part by confocal microscopy (Extended Data Fig. 2d). The gene discussed is SELPLG; the disease is HIV-1 infection.