CD4 and HIV-1 infection: As such, we targeted the expression of five genes with roles in HIV-1 infection (CD4, CXCR4, PSGL-1, TRIM5α and CPSF6) as well as CD46, in part using gRNAs pre-tested for each target for the efficiency of editing (Extended Data Fig. 4a) and protein loss was assessed in part by confocal microscopy (Extended Data Fig. 2d).