Table 8 and Table 9 showed the number of transcripts aligned by both tools taking unique transcripts across the samples and total transcripts across the samples, respectively. Consistent with the lung cancer case results, as shown in Table 10, Kallisto aligned more transcripts for liver cancer data as well. The biomarker gene SALL4, which is localized in the nucleus, had a low fold change value. Table 11 shows a comparison of the tools in finding the biomarkers based on high fold change. The gene discussed is SALL4; the disease is lung cancer.