This study focuses on the prostate cancer cell line DU145 and the ovarian cancer cell line IGROV1, as both cell lines have been previously reported to show reduced cell proliferation and tumor growth in xenograft models upon TUT4 transcript depletion via siRNAs or shRNAs (Piskounova et al. 2011; Fu et al. 2014). Here, TUT4 is linked to Familial prostate cancer.