Additionally, results of the tumor spheroid assay revealed that LY2228820 suppressed cell sphere formation, as shown in Fig. 6C. As shown in Fig. 6D (P < 0.05), Bach1, CD44, and p-p-38 protein expression triggered by LV-Bach1 was greatly reduced by LY2228820 in A549 CSCs. The gene discussed is BACH1; the disease is neoplasm.