The shift in the storage of lipids in adipose tissue to storage in ectopic tissues was dependent on the duration of HFD and occurred with the onset of systemic insulin resistance shown by the increase in the area under the ITT, the expression of adipose tissue insulin resistance markers (Socs3 and Notch1 mRNA), the inability of fat depots to increase in size, and the presence of crown cells, which indicated dysfunctionality in SAT and EPI depots. The gene discussed is NOTCH1; the disease is Insulin resistance.