NOTCH2 and melanoma: In addition, gliotoxin can also induce apoptosis via the deregulation of NOTCH2 mRNA expression towards cell lines derived from melanoma (518A2), hepatocellular carcinoma (SNU398, HCC-3, Hep3B), and pancreas carcinoma (PANC1) with the high expression level of NOTCH2; the apoptotic effect was not observed in the NOTCH-negative cell line Huh7 [87], suggesting the vital role of NOTCH in gliotoxin-inducing apoptosis.