As shown in Figure 9, the expression of nephrin and podocine did not change between WT and APC1638T mice in the normal condition, but they were reduced in both mice after PAN-induced nephrotic syndrome induction, merely reflecting the reduction in the number of slit membranes by foot process effacement that was observed to the same extent in both mice (Figure 5). Here, NPHS2 is linked to nephrotic syndrome.