The optimized NE system loaded with ETO was then investigated for its anticancer potential, employing a A549 lung cancer cells line via cytotoxicity, cell migration, apoptotic activity, mitochondrial membrane potential (MMP) activity, cell cycle analysis, Caspase-3, 9, and p53 activity by ELISA and molecular marker analysis through RT-PCR analysis. Here, TP53 is linked to lung carcinoma.