TTC5 and osteosarcoma: The H2170 and A549 lung cancer cell line, osteosarcoma cell lines (U2OS), as well as BEAS 2B noncancerous lung cell line were treated for 24 h with 20 μM Etoposide, the cells were fixed and nuclei stained by DAPI (blue), P53 specific DO-1, anti-K382, and anti-TTC5 antibodies (Supplementary Figures S4–S7).