PRKDC and central nervous system cancer: While DNA-PKcs-proficient glioma M059K cells were found to be prone to lentiviral transduction at a rate of 22% of positively transduced cells, its DNA-PKcs-deficient counterpart, M059J, was profoundly defective in lentivirus transduction, and only 8.5% of the cell population was infected (untreated M059K vs. untreated M059J, p < 0.0001; Figure 3C).