Although several HDM allergens, -i.e., Der p 5, Der p 7, and the peritrophin-like domain (Der p 21 and Der p 23)- have been quantified as serodominant molecules in the present study, their actual allergenic activity would be only confirmed if the IgE-binding molecules accomplished one positive in vivo and in vitro provocation tests in humans, a positive association with an allergic disease in case–control studies, and a specific proinflammatory pathogenic mechanism [32]. Here, IGHE is linked to allergic disease.