The A549 human lung cancer cell line and the MCF-7 breast cancer cell line (luminal A, estrogen receptor positive) were seeded at 6 × 103 cells/cm2, serum starved and treated with TGF-β (10 ng/mL) and with or without different doses of MDZ (5, 10, and 20 μM). Here, TGFB1 is linked to lung carcinoma.