In the three CAF isolates from HCC derived from patient 1–3 cancer stem cells (CSC) positive for CD13 [48,49], CD44, CD90 [49], CD133 [50], OV6 [49], epithelial cells or CSCs positive for EpCAM [49], or general tumor cells positive AFP [51] were identified by immunofluorescence. This evidence concerns the gene AFP and hepatocellular carcinoma.