Thereby, we analysed caspase cascade signalling in the GBM cell line A172 and a well-characterised TRAIL-responsive human colon cancer cell line HCT116 in response to equimolar amounts of the TRAIL trimer, i.e., 1 nM hexavalent scTRAIL-Fc-ANG2 or 2 nM trivalent scTRAIL-ANG2. Here, TNFSF10 is linked to colonic neoplasm.