For the inflammatory factors, as shown in Figure 6a, the relative expression of inflammatory factors TNF-α, IL-1β and IL-6 in NAFLD group increased significantly, reaching 5.3 ± 1.87 times, 53.1 ± 10.89 times and 32.45 ± 13.8 times of the normal group, respectively. Here, TNF is linked to metabolic dysfunction-associated steatotic liver disease.