In subsequent years, several selection criteria were developed that, in addition to the morphological data (number and size of nodules), took into consideration surrogate markers of tumour aggressiveness, such as alpha-fetoprotein (AFP), HCC histological grading, the neutrophil/lymphocyte ratio (NLR), and the behaviour of HCC on PET-CT (Figure 1) [12,13,14,15,16,17,18,19,20,21]. This evidence concerns the gene AFP and neoplasm.