The first one, a low-pH medium, referred to as LSA, is an Acidified hyperglycemic Low-Serum medium (pH 6.6, 50 mM glucose, 2.5% foetal bovine serum) that boosts the invasive potential of cancer cells (Supplementary Figure S3); the second, here referred to as H2O2 (tested in two different serum conditions: High Serum (HS containing 10% foetal bovine serum) or Low Serum (LS containing 2.5% foetal bovine serum)), is an oxidative stress medium (DMEM with 10% or 2.5% FBS at normal pH 7.4 with 25 mM glucose and 100 μM H2O2) that induces cellular insult and leads to p53 activation (Figure S4). Here, TP53 is linked to cancer.