In conclusion, we used absolute real-time PCR to demonstrate U50A as a better prognostic marker in breast cancer (Figure 1) and found that U50A downregulates the mitosis-related genes SMC5, ATRX, CENPE, and CENPF, which arrest cells in prometaphase, leading to prolonged M phase and downregulated clonogenic activity (Figure 2, Figure 3 and Figure 4). Here, CENPF is linked to breast cancer.