PTPRC and neoplasm: Analysis of the immune infiltrate was performed with multiparametric flow cytometry, and a representative example of the gating strategy used is reported in Figure 1A. After doublets exclusion and gating on live cells, we first determined the leukocyte cell abundance by analyzing the percentage of CD45+ cells (Figure 1A) and found a median ranging from 24.8% to 38.6% of live cells across the tumor samples, without significant differences between diagnosis and relapses in either of the tumor areas (Figure 1C).