Interestingly, the top up-regulated pathways in the M3 iSubtype were mainly involved in immune system (allograft rejection, interferon alpha/gamma response, inflammatory response, complement (part of the innate immune system)), signaling transduction (IL6/JAK/STAT3, TNFA signaling via NFKB, KRAS, IL2_STATS, NOTCH), cell cycle (E2F targets, G2/M checkpoint) and tumor progression (epithelial-to-mesenchymal transition, angiogenesis, coagulation, hypoxia). The gene discussed is TNF; the disease is neoplasm.