Using RT-PCR, the Percoll-purified microglia fraction from 20 human GBM specimens was analyzed for the gene expression of key cytokines and chemokines (encoding EGF, PDGFα, PDGFβ, NGF, IL-6, IL-8, IL-10, CXCR12, and CCL5). This evidence concerns the gene CXCL8 and glioblastoma.