To identify the microglial factors activating Pyk2 and FAK signaling in glioma cells, the phosphorylated (p-Pyk2 (Y579/Y580) and p-FAK (Y925)) and total protein levels of these kinases were evaluated upon cell treatment with MCM or with each cytokine/chemokine identified above (EGF, PDGFα, PDGFβ, SDF-1α, IL-6, and IL-8). This evidence concerns the gene PTK2B and central nervous system cancer.