Herein, we measured the levels of sPD-1 in plasma collected from patients with NSCLC, gastric cancer, and bladder cancer who underwent anti-PD-1 antibody therapy, and analyzed the change in sPD-1 expression from diagnosis to posttreatment to investigate the clinical implications of sPD-1 level measurement in patients with cancer. This evidence concerns the gene HOXD13 and non-small cell lung carcinoma.