For example, a high-fat diet (HFD)-induced development of NAFLD has been reported to be associated with a decrease in the ratio of non-12α-OH BAs (e.g., HDCA/Hyodeoxycholic)/12α-OH BAs (e.g., DCA) with downregulation of FXR and TGR5 and upregulation of cytochrome P450 family 7 subfamily A member 1 (CYP7A1) and TLR4 [28]. Here, NR1H4 is linked to metabolic dysfunction-associated steatotic liver disease.