Percentages of Th1 (CD3+ CD4+ CXCR3+ CCR6-), Th2 (CD3+ CD4+ CXCR3- CCR6-), Th17 (CD3+ CD4+ CXCR3- CCR6+) and Treg (CD4+ CD25+ CCR4+ CD45RO+) cells were analyzed in PBMC cultures from RRMS patients and HD, after 5 days of co-culture. The gene discussed is CD4; the disease is relapsing-remitting multiple sclerosis.