TGFB1 and metabolic dysfunction-associated steatohepatitis: Indeed, TGF-β-mediated activation of both primary and immortalized HSCs (LX-2) was also observed with F4/80+ BMDMs, which were present in the liver tissue of different animal models such as high fat and high cholesterol (HFHC) model or a methionine-choline deficient (MCD) diet used to study non-alcoholic steatohepatitis (NASH)-related fibrosing steatohepatitis.