In human-induced pluripotent stem-cell-derived cardiomyocytes stimulated with endothelin-1 (ET-1), miR-19a, miR-21, miR-29b and miR-199 are overexpressed, generating cardiac hypertrophy in vitro, however, when the analysis was performed on circulating miRNAs in the serum of chagasic patients with LVH, only miR-19a, miR-21 and miR-29b were overexpressed [43]. The gene discussed is EDN1; the disease is cardiac hypertrophy.