AKT1, SRC, EGFR, and GSK3B were identified as key anti-obesity target genes of hispidulin, and estrogen, prolactin, Rap1, and PI3K-Akt signaling pathways were predicted to be involved in the anti-obesity effects of hispidulin. The gene discussed is EGFR; the disease is obesity due to melanocortin 4 receptor deficiency.