We also investigated the gene and protein expression of several hypertrophic markers on the EHTs cultured for at least 14 days on stiffer posts, and no differences were observed between normal and stiffer posts on both control and PAH EHTs (Figure 7C,D), except trends for reduced ATP2A2 gene expression (p = 0.078), reduced NPPB gene expression (p = 0.065), and reduced MYH7 gene expression (p = 0.079) on stiffer posts compared with normal posts in PAH EHTs. The gene discussed is MYH7; the disease is pulmonary arterial hypertension.