It was shown that exposure of naive murine macrophages to such exosomes released from preheated breast cancer 4T1 and EMT-6 cells resulted in an increased expression of specific macrophage activation markers [235]; this finding suggests the immunogenic potential intrinsic in the HSP70-containing exosomes released from breast cancer cells treated with mild hyperthermia. Here, HSPA1A is linked to breast carcinoma.