In addition, we used mice with ectopic expression of agouti protein as a model of melanocortin obesity; however, this ectopic expression may result in blockade of melanocortin receptors, including MC1R and MC2R, not only in the brain, but also in the periphery, introducing some phenotypic features specific to this model. Here, MC2R is linked to obesity due to melanocortin 4 receptor deficiency.