Conversely, iPSC-derived dopaminergic neurons from patients carrying GBA1 mutations (N370S, L444P, and RecNcil) showed increased basal and caffeine-induced cytosolic Ca2+ levels, indicating impaired mitochondrial Ca2+ buffering and ER-mitochondrial Ca2+ transfer Additionally, LRRK2 G2019S PD patient neurons showed elevated intracellular Ca2+, possibly due to LRRK2 hyperactivity upregulating translation of Ca2+ signaling proteins [176]. This evidence concerns the gene LRRK2 and Parkinson disease.