Alpha-fetoprotein (AFP) is an important serological marker for early screening of HCC, but since about 30–40% of HCC patients are clinically negative for AFP [7], it is easy to misdiagnose AFP-negative patients as other liver tumors, so early and accurate preoperative diagnosis and differentiation of AFP-negative HCC from OM are particularly important for the selection of clinical treatment options and improvement of prognosis [8]. The gene discussed is AFP; the disease is ocular melanoma.