As expected (based on protective effects of prior vaccination suppressing disease manifestation), sensitivity increased when only serum samples from unvaccinated control animals after a single dose infection and postinfection follow-up of at least two months were considered, generating ROC-AUC values of 0.87, 0.95, 0.82 and 0.91 for SAA1, IP-10, IL-6, and 3BM, respectively (Table 1b). Here, SAA1 is linked to infection.