GFAP and infection: The observed reduction in numbers of GFAP+ cells in rAAV-shNCLX-infected cultures is unlikely to reflect an impairment of glial cell proliferation, since the proportion of GFAP+ cells in sister cultures infected with rAAV-shCTRL either was decreased (7 × 108 viral particles/ml infection: DIV 8 17.74 ± 2.57%; DIV 10 13.70 ± 2.74%; two-tailed paired-samples t test; t(5) = 3.831, p = 0.0122) or was unchanged in this time frame (2 × 109 viral particles/ml infection: DIV 8 22.21 ± 1.85%; DIV 10 22.00 ± 1.62%; two-tailed paired-samples t test; t(2) = 0.6310, p = 0.5926).