In keeping with the idea that NCLX knockdown leads to astrocytic cell death, we found a significantly smaller proportion of viable GFAP+ cells on DIV 10 for both our standard infection rate (Fig. 4C) and for the three-times greater infection rate (Fig. 4, D and E) in cultures that had been infected with rAAV-shNCLX compared with those infected with rAAV-shCTRL. The gene discussed is SLC8B1; the disease is infection.