Quantitative reverse transcription polymerase chain reaction (qRT–PCR) analysis revealed that, compared with a control shRNA sequence with no known targets in the mouse genome (shCTRL) (27), rAAV-mediated expression of NCLX-directed shRNA effectively reduced Nclx RNA levels in primary hippocampal cultures starting as early as 5 days after infection, on day in vitro (DIV) 8 (Fig. 1B). This evidence concerns the gene SLC8B1 and infection.