More specifically, we designed shRNA sequences against mouse Nclx (shNCLX-1, also referred to as shNCLX, and shNCLX-2) and cloned these behind the U6 small nuclear RNA promoter of an rAAV expression cassette that also drives expression of the red fluorescent protein mCherry in excitatory neurons via the calcium/calmodulin-dependent protein kinase II alpha (CaMK2a) promoter as an infection marker (Fig. 1A). Here, CAMK2A is linked to infection.