The comparison V (Figure 1A) of n = 26 IDH1-mut astrocytoma WHO grades II/III with n = 7 IDH1-mut glioblastoma (WHO grade IV) uncovered 15 significant metabolites in the 2-tailed t test (Tables 1 and 2), including ATP, butyrate, citrate, formate, fumarate, glutamate, glutathione, histamine, histidine, lactate, o-acetylcholine, phenylalanine, succinate, taurine, and uridine. The gene discussed is IDH1; the disease is astrocytoma (excluding glioblastoma).