By comparison VII (Figure 1A) (n = 9 IDH1-WT astrocytoma WHO grades II/III versus n = 30 IDH1-WT glioblastoma), we identified 9 significant metabolites in the 2-tailed t test (4-aminobutyrate, alanine, ascorbate, butyrate, creatine, creatine phosphate, hypoxanthine, myo-inositol, and scyllo-inositol) (Tables 1 and 2) and 11 PLS-DA variables with VIP scores > 1 (Figure 6C). Here, IDH1 is linked to astrocytoma (excluding glioblastoma).