Joint pathway and interaction analysis of differentially expressed proteins and metabolites revealed crosstalk among potential candidates such as valproic acid, leucic acid, 4-hydroxy-2-oxoglutaric acid, F2, glycerol, SERPING1, KNG1, FN1, BHB, CP, and C3, which could be responsible for restoring associated complication of NEB such as infertility, or infection such as mastitis. This evidence concerns the gene C3 and Infertility.