Current researchers still use the procedures developed by Choi et al. (2014) to model AD in vitro; For instance, Kwack et al. [91] recently used the hNPC-generation protocol established by Choi et al. to confirm that Aβ42/40 ratio, not overall Aβ level, correlates with tau pathology in 3D cell culture models of AD. Here, MAPT is linked to Alzheimer disease.