Greening and coworkers (2015) [12] evaluated the efficacy of three isolation techniques: ultracentrifugation (UC-Exos), OptiPrepTM density gradient centrifugation (DG-Exos), and immune isolation using EpCAM (CD326) antibodies coupled to magnetic beads (IAC-Exos) targeting markers Alix, TSG101, and HSP70 to enrich exosomes released from LIM1863 human colon cancer cells. This evidence concerns the gene EPCAM and malignant colon neoplasm.