To further validate the expression of these candidate biomarkers in ulcerative colitis, we determined mRNA levels of SGK1, CEP55, ACSL1, OLFM4, and DPP10 in lipopolysaccharides (LPS)‐stimulated Raw264.7 cells by quantitative reverse transcription‐polymerase chain reaction (qPCR) in in vitro bioassays. This evidence concerns the gene ACSL1 and ulcerative colitis.