The results of the present study indicate that all three null hypotheses have to be rejected, as it was demonstrated that the size of AgNPs affected the response of the tested human osteosarcoma cell lines, the concentration of AgNPs affected the response of the tested human osteosarcoma cells, and finally the presence of protein p53 affected the response of osteosarcoma cells to AgNP treatment. Here, TP53 is linked to osteosarcoma.