In addition, we performed a subgroup survival analysis of OS with TCGA dataset, and we found that high expression of MK3 was associated with poor prognosis in WHO grade G3, non-codel 1p/19q codeletion, age less than or equal to 60 years, age greater than 60 years, and histological type astrocytoma subgroup of glioma (Figures 3G–O). This evidence concerns the gene MAPKAPK3 and astrocytoma (excluding glioblastoma).