We firstly construct a miRNA-mRNA network in stroke and demonstrate the potential signaling pathways (hsa-miR-3591-5p/CTNNB1, hsa-miR-548as-3p/PTEN, hsa-miR-4503/MYCN, hsa-miR-206/ESR1, and hsa-miR-206/AKT1) regulating pathological mechanisms of stroke. This evidence concerns the gene ESR1 and stroke disorder.