CD8A and neoplasm: Pairwise comparison of baseline and on-treatment multiplex-stained primary tumor samples could be performed in 26 patients (Supplementary Fig. 5c) and revealed a significant increase in intratumoral CD3 + CD8 + T-cell density in both MPR and non-MPR patients after ICB (P = 0.04 and P = 0.0003, respectively, Supplementary Fig. 5d).