Considering the potential impact of infected donor cells on cell-to-cell transmission, we normalized the rate of cell-to-cell transmission with the total rate of virus spread in both SARS-CoV-2–positive Vero-mTomato-Red cells as well as Vero-ACE2 cells over the entire infection period, i.e., from the initial infection of donor cells to the end of the coculture (∼26 h). Here, ACE2 is linked to infection.