After the replacement of HFD with SD for 2 months, the T2D/WL group lost significant weight (p < 0.001), reduced fasting glycemia (p < 0.0001) and improved insulin sensitivity (p < 0.05) compared to the mice that were kept on HFD (Fig. 1d–f, T2D/Ob mice). This evidence concerns the gene INS and type 2 diabetes mellitus.