Previous studies have indicated that there is dysregulation in the early endosomal trafficking in different Huntington's disease cell lines.54,55 Moreover, Huntington's disease pathogenesis in some mouse models has been linked to decreased Rab11 activity in recycling endosomes.56 We therefore analysed different endosomal proteins to discover whether there were any subcellular alterations in endosomal trafficking using high content automated microscopy. Here, RAB11A is linked to juvenile Huntington disease.