This identified 34 AD risk loci, including 4 novel loci near SPRED2, NCK2, CCDC6, and TSPAN14. Two additional novel loci achieved subthreshold GWS: TMEM163 on chromosome 2 (p = 5.24E − 08) and IKZF1 on chromosome 7 (p = 7.68E − 08). This evidence concerns the gene NCK2 and Alzheimer disease.