Previous studies reported the observation that the volume of tissue damage in the middle cerebral artery occlusion (MCAO) ischemic stroke model was greater in the Cx43(Δ/−) mouse13 and that in the dKO mouse brain high pressure perfusion (HPP) led to greater vascular leakage of HRP than in the wildtype mouse8. Here, GJA1 is linked to ischemic stroke.